High cell density cultivation of a recombinant E. coli strain expressing a key enzyme in bioengineered heparin production
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چکیده
منابع مشابه
High cell density cultivation of a recombinant Escherichia coli strain expressing a 6-O-sulfotransferase for the production of bioengineered heparin.
AIMS One of six heparin biosynthetic enzymes, cloned and expressed in Escherichia coli as a soluble fusion protein, requires large-scale preparation for use in the chemoenzymatic synthesis of heparin, an important anticoagulant drug. METHODS AND RESULTS The 6-O-sulfotransferase isoform-3 (6-OST-3) can be conveniently prepared at mg/L levels in the laboratory by culturing E. coli on Luria-Bert...
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Bioengineered heparin is being investigated as a potential substitute for the animal-sourced anticoagulant drug. One step in the current process to prepare bioengineered heparin involves the conversion of N-sulfo heparosan, rich in → 4)GlcNS(1 → 4) GlcA(1 → sequences (where S is sulfo, GlcN is α-D-glucosamine, and GlcA is β-D-glucuronic acid), to a critical intermediate, rich in → 4)GlcNS(1 → 4...
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ژورنال
عنوان ژورنال: Applied Microbiology and Biotechnology
سال: 2013
ISSN: 0175-7598,1432-0614
DOI: 10.1007/s00253-012-4682-z